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 DeCS Categories

D08 Enzymes and Coenzymes .
D08.811 Enzymes .
D08.811.150 DNA Restriction-Modification Enzymes .
D08.811.150.280 DNA Restriction Enzymes .
D08.811.150.280.250 Deoxyribonucleases, Type I Site-Specific .
D08.811.150.280.260 Deoxyribonucleases, Type II Site-Specific .
D08.811.150.280.260.300 Deoxyribonuclease EcoRI .
D08.811.277 Hydrolases .
D08.811.277.352 Esterases .
D08.811.277.352.335 Deoxyribonucleases .
D08.811.277.352.335.350 Endodeoxyribonucleases .
D08.811.277.352.335.350.300 DNA Restriction Enzymes .
D08.811.277.352.335.350.300.250 Deoxyribonucleases, Type I Site-Specific .
D08.811.277.352.335.350.300.260 Deoxyribonucleases, Type II Site-Specific .
D08.811.277.352.335.350.300.260.250 Deoxyribonuclease EcoRI .
D08.811.277.352.355 Endonucleases .
D08.811.277.352.355.325 Endodeoxyribonucleases .
D08.811.277.352.355.325.300 DNA Restriction Enzymes .
D08.811.277.352.355.325.300.250 Deoxyribonucleases, Type I Site-Specific .
D08.811.277.352.355.325.300.260 Deoxyribonucleases, Type II Site-Specific .
D08.811.277.352.355.325.300.260.250 Deoxyribonuclease EcoRI .
E05 Investigative Techniques .
E05.393 Genetic Techniques .
E05.393.183 Chromosome Mapping .
E05.393.183.620 Physical Chromosome Mapping .
E05.393.183.620.650 Restriction Mapping .
E05.393.712 Restriction Mapping .
 
 Terms
 Synonyms & Historicals
Documents
LILACS e MDL
 
DNA Restriction Enzymes .
DNA Restriction Enzyme .
Restriction Endonuclease .
Endonuclease, Restriction .
Endonucleases, Restriction .
Enzymes, DNA Restriction .
Restriction Enzyme, DNA .
Restriction Enzymes, DNA .
Restriction Endonucleases .
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1. .
1.00
6322541
 
Deoxyribonuclease EcoRI .
Eco RI .
Eco-RI .
EcoRI Endonuclease .
Endodeoxyribonuclease ECoRI .
Endodeoxyribonuclease HsaI .
Endonuclease Eco159I .
Endonuclease Eco82I .
Endonuclease RsrI .
Endonuclease SsoI .
HsaI Endonuclease .
Restriction Endonuclease RsrI .
Deoxyribonuclease SsoI .
DNA Restriction Enzyme EcoRI .
Endonuclease EcoRI .
One of the Type II site-specific deoxyribonucleases (EC 3.1.21.4). It recognizes and cleaves the sequence G/AATTC at the slash. EcoRI is from E coliRY13. Several isoschizomers have been identified. EC 3.1.21.-. .
0.80
52020
 
Restriction Mapping .
Analysis, Restriction Enzyme .
Enzyme Analysis, Restriction .
Restriction Enzyme Analysis .
Analyses, Restriction Enzyme .
Endonuclease Mappings, Restriction .
Enzyme Analyses, Restriction .
Enzyme Mappings, Restriction .
Mapping, Restriction .
Mapping, Restriction Endonuclease .
Mapping, Restriction Enzyme .
Mapping, Restriction Site .
Mappings, Restriction .
Mappings, Restriction Endonuclease .
Mappings, Restriction Enzyme .
Mappings, Restriction Site .
Restriction Endonuclease Mapping .
Restriction Endonuclease Mappings .
Restriction Enzyme Analyses .
Restriction Enzyme Mapping .
Restriction Enzyme Mappings .
Restriction Mappings .
Restriction Site Mapping .
Restriction Site Mappings .
Site Mappings, Restriction .
Endonuclease Mapping, Restriction .
Enzyme Mapping, Restriction .
Site Mapping, Restriction .
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA. .
0.78
3336496
 
Deoxyribonucleases, Type I Site-Specific .
DNase, Site Specific, Type I .
Deoxyribonucleases, Type I, Site Specific .
Deoxyribonucleases, Type I, Site-Specific .
Site-Specific DNase, Type I .
Type I Site Specific DNase .
Type I Site Specific Deoxyribonucleases .
Type I Site-Specific DNase .
Type I Site-Specific Deoxyribonucleases .
Deoxyribonucleases, Type I Site Specific .
Site Specific DNase, Type I .
DNA Restriction Enzymes, Type I .
Restriction Endonucleases, Type I .
Type I Restriction Enzymes .
DNase, Site-Specific, Type I .
Enzyme systems containing three different subunits and requiring ATP, S-adenosylmethionine, and magnesium for endonucleolytic activity to give random double-stranded fragments with terminal 5'-phosphates. They function also as DNA-dependent ATPases and modification methylases, catalyzing the reactions of EC 2.1.1.72 and EC 2.1.1.73 with similar site-specificity. The systems recognize specific short DNA sequences and cleave at sites remote from the recognition sequence. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC 3.1.21.3. .
0.77
5224
 
Deoxyribonucleases, Type II Site-Specific .
DNase, Site Specific, Type II .
Deoxyribonucleases, Type II, Site Specific .
Deoxyribonucleases, Type II, Site-Specific .
Site-Specific DNase, Type II .
Type II Site Specific DNase .
Type II Site Specific Deoxyribonucleases .
Type II Site-Specific DNase .
Type II Site-Specific Deoxyribonucleases .
Deoxyribonucleases, Type II Site Specific .
Site Specific DNase, Type II .
DNA Restriction Enzymes, Type II .
Type II Restriction Enzymes .
DNase, Site-Specific, Type II .
Restriction Endonucleases, Type II .
Enzyme systems containing a single subunit and requiring only magnesium for endonucleolytic activity. The corresponding modification methylases are separate enzymes. The systems recognize specific short DNA sequences and cleave either within, or at a short specific distance from, the recognition sequence to give specific double-stranded fragments with terminal 5'-phosphates. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC 3.1.21.4. .
0.77
148319