Oligonucleotide Probes. Probes, Oligodeoxyribonucleotide . Probes, Oligonucleotide . Probes, Oligoribonucleotide . Oligodeoxyribonucleotide Probes . Oligoribonucleotide Probes . Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin. . 1.00
DNA Probes. Conserved Gene Probes . DNA Hybridization Probes . Whole Chromosomal Probes . Whole Genomic DNA Probes . Chromosomal Probes, Whole . DNA Gene Probes . Gene Probes, Conserved . Hybridization Probes, DNA . Probes, Chromosomal . Probes, Conserved Gene . Probes, DNA . Probes, DNA Gene . Probes, DNA Hybridization . Probes, Whole Chromosomal . Chromosomal Probes . Gene Probes, DNA . Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections. . 0.79
RNA Probes. Probes, RNA . Probes, RNA Gene . RNA Gene Probes . Gene Probes, RNA . RNA, usually prepared by transcription from cloned DNA, which complements a specific mRNA or DNA and is generally used for studies of virus genes, distribution of specific RNA in tissues and cells, integration of viral DNA into genomes, transcription, etc. Whereas DNA PROBES are preferred for use at a more macroscopic level for detection of the presence of DNA/RNA from specific species or subspecies, RNA probes are preferred for genetic studies. Conventional labels for the RNA probe include radioisotope labels 32P and 125I and the chemical label biotin. RNA probes may be further divided by category into plus-sense RNA probes, minus-sense RNA probes, and antisense RNA probes. . 0.43
Chromosome Painting. Chromosome Paintings . Painting, Chromosome . Paintings, Chromosome . A technique for visualizing CHROMOSOME ABERRATIONS using fluorescently labeled DNA probes which are hybridized to chromosomal DNA. Multiple fluorochromes may be attached to the probes. Upon hybridization, this produces a multicolored, or painted, effect with a unique color at each site of hybridization. This technique may also be used to identify cross-species homology by labeling probes from one species for hybridization with chromosomes from another species. . 0.41
Nucleic Acid Probes. Acid Probes, Nucleic . Probes, Nucleic Acid . Nucleic acid which complements a specific mRNA or DNA molecule, or fragment thereof; used for hybridization studies in order to identify microorganisms and for genetic studies. . 0.39
